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Accession IconSRP047246

Expression profiling pooled Drosophila melanogaster heterozygous for deletions on Chromosome 2L

Organism Icon Drosophila melanogaster
Sample Icon 343 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We performed mRNA transcriptional profiling on 99 hemizygotic lines (Df/+) from the DrosDel project covering 68% of chromosome 2L, in order to understand how changes in gene copy number affect overall transcriptome. Overall design: We performed RNA-Seq analysis on 396 pools of 15-25 adult flies each. Samples include males or females from 99 different genotypes in duplicate. Differential gene expression was analyzed separately for each sex, by comparing each genotype with the remaining 98.
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396
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