3'' RNA-seq of fly expressing cabut RNAi after exposure to different levels of sucrose

Control (+/cbtE1-UAS-cbt RNAi) or cabut RNAi flies (Tim-gal4, UAS-cbt RNAi) were starved for 16 hours and then exposed to food containing different concentrations of sucrose: 0, 25, 50 and 100 % for 18 hours. Fly heads were collected, RNA was extracted and RNA-seq libraries were prepared as previously described (Engreitz et al., 2013) Overall design: For each sucrose concentration, two samples of cabut RNAi flies and one sample of control flies were sequenced.

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Bartok O, Teesalu M, Ashwall-Fluss R, Pandey V, Hanan M, Rovenko BM, Poukkula M, Havula E, Moussaieff A, Vodala S, Nahmias Y, Kadener S, Hietakangas V