Illumina HiSeq 2500 paired end sequencing; GSM1375518: NT-1; Homo sapiens; RNA-Seq

NT-1

A new method to measure elongation and intitiation rates Overall design: Reversal inhibition of transcription with DRB and tagging newly transcribed RNA with 4-thiouridine (4sU)

4sUDRB-seq: measuring transcription elongation and initiation genomewide

Submitted by Gene Expression Omnibus on 13-MAY-2014

4sU labled RNA was tagged with Biotion, and enriched with Streptavidin magnetic beads. cDNA libraries were prepared with Illumina TruSeq RNA sample preparation v2 kit according to the manufacturer's instructions but without the polyA isolation stage

4sUDRB-seq: measuring transcription elongation and initiation genomewide

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Samples