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Accession IconSRP038922

RNA-seq study of major P.aeruginosa PA14 transcription factors

Organism Icon Pseudomonas aeruginosa
Sample Icon 14 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500, Illumina Genome Analyzer IIx

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Description
Gene regulation via transcription factors influences the metabolic, adaptive and pathogenic capabilities of the organism. We report the transcriptomes of the mutants of six major P. aeruginosa PA14 trancription factors - RhlR, LasR, Anr, GacA, FleQ and CbrB. Overall design: The P. aeruginosa PA14 transposon mutants were analyzed by RNA-seq. All samples were cultivated in LB medium until reaching an OD600 of 2.0. For each biological replicate, three cultures were pooled for RNA extraction, library preparation and sequencing.
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14
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