Mus musculus musculus Transcriptome or Gene expression

Hair-related diseases are a major public-health problem, and their treatments are limited to hormone-therapies. Researchers have tried to investigate the genes and signal pathways underlying hair follicle (HF) stem cells and interaction of HFs and dermal papilla cells (DPCs) for specific targeted-therapies through microarrays, appropriate for the analysis of small genomes. Therefore, to enable a comprehensive transcriptome analysis for the large, complex transcriptomes, we performed RNA-seq using next generation sequencing (NGS). We isolated interfollicular keratinocytes (IFKs), HFs, and DPCs form C57BL/6 murine skin, transplanted combinations of these samples into nude mice, and followed up. Sustained hair growth was supported by intact HFs and DPCs. And, we identified 19 HF- and 34 DPC-specific novel genes with NGS, validated these data with quantitative real-time RT-PCR, and performed pathway analysis of these genes. In addition, HFs had a more quiescent cell-cycle pattern than did IFKs and DPCs in culture and flow cytometry (FCM). Therefore, the representative cell cycle-related gene expression in IFKs, HFs and DPCs were analyzed by NGS. These genes will allow the investigation of the interactions and signaling pathways involved in HF-related diseases and cancer, support bioengineering, and may be used as specific and novel markers of these cell types.

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