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Accession IconSRP028570

Differential transcript stability measurements in MDA-MB-231 vs. MDA-LM2 cells

Organism Icon Homo sapiens
Sample Icon 32 Downloadable Samples
Technology Badge IconIllumina HiSeq 2000

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Description
We performed whole-genome stability measurements for MDA-MB-231 and its highly metastatic derivative MDA-LM2. Our goal was to identify post-transcriptonal regulons that are deregulated en route to higher metastatic capacity. Overall design: Cells were pulsed with 4-thiouridine for 2 hours and then RNA was extracted at 0, 2, 4, and 7 hr time-points in quadruplicate from each cell line. 4sU labeling followed by RNA-seq was then used to measure the abundance of transcripts in each population. A decay rate was estimated based on the rate at which transcript abundance was reduced at these time-points.
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32
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