Illumina HiSeq 2000 paired end sequencing; GSM1126612: NF1_RNASeq; Homo sapiens; RNA-Seq

NF1_RNASeq

Complete transcriptome profiling in human failing and non-failing control hearts using next-gen sequencing Overall design: Poly-A selected RNA and small RNA sequencing carried out in 5 groups of samples: NF, ICM, NICM, ICM+LVAD, NICM+LVAD

Deep RNA Sequencing Reveals Dynamic Regulation of Myocardial Noncoding RNA in Failing Human Heart and Remodeling with Mechanical Circulatory Support

Submitted by Gene Expression Omnibus on 17-FEB-2014

LV apex tissues were collected, incubated in RNAlater for 24 hours then frozen in -80â„ƒ; RNA were extracted using TriZol, RNA quality confirmed with Qbit (RIN>8) RNA libraries were prepared using Illumina TrueSeq RNA Sample Prep Kits; small RNA libraries were prepared using Illumina TrueSeq Small RNA Sample Prep Kits

Deep RNA Sequencing Reveals Dynamic Regulation of Myocardial Noncoding RNA in Failing Human Heart and Remodeling with Mechanical Circulatory Support

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Samples