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Accession IconSRP009270

MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]

Organism Icon Mus musculus
Sample Icon 12 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer IIx, Illumina Genome Analyzer II

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Description
Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts. Overall design: MIWI-associated piRNAs from different genotypes were sequenced. Total RNA from purified round spermatids were subjected to Ribozero purification and strand-specific RNAseq lib prepared. Global 5'' RACE library was prepare from indicated genotypes.
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12
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