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Accession IconSRP007487

MicroRNA profiling of murine T lymphopoiesis

Organism Icon Mus musculus
Sample Icon 12 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer II

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Description
Here we describe microRNA profiling of a single differentiation pathway from the stem cell through to terminally differentated mature cells. Overall design: Populations corresponding to distinct stages in T lymphocyte development, from the hematopoietic stem cell-enriched Lin-Sca+Kit+ population through to mature CD4+ and CD8+ T cells were FACS-sorted to purity from the bone marrow and thymus of C57BL/6 mice. Total RNA was extract from each population from which microRNA sequencing libraries were constructed.
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12
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