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Accession IconSRP006898

Expression profile of 16C ovarian follicle cells

Organism Icon Drosophila melanogaster
Sample Icon 2 Downloadable Samples
Technology Badge IconIllumina Genome Analyzer II

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Description
We use DSN normalized RNA-seq to transcriptionally profile FACS sorted 16C ovarian follicle cells. These data provide insights into the developmental control of gene expression programmed gene amplificaton. Overall design: Follicle cells were isolated from whole ovaries by trypsinization and filtering and stained with Hoescht. 16C follicle cells were isolated by FACS sorting based on DNA content (Hoescht). RNA was extracted with TRIzol reagent and 100ng of total RNA and used to generate a total library. This library was then subjected to DSN normalization prior to Illumina based sequencing.
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