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Accession IconSRP002309

microRNA-target RNA pairs revealed by Parallel analysis of RNA ends in Vitis vinifera

Organism Icon Vitis vinifera
Sample Icon 1 Downloadable Sample
Technology Badge IconIllumina Genome Analyzer

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Description
Vitis vinifera RNA degradome Overall design: Isolated polyadenylated RNA from total RNA extracts of Vitis vinifera leaves, were ligated to 5'-adapter that include san MmeI recognition site. The ligated products were purified again, reverse transcribed and cleaved with MmeI. The 5' fragments were purified from gel and to a 3'- dsDNA adapter and PCR amplified. After PCR amplification the sample was subjected to Solexa/Illumina high throughput pyrosequencing. Please see www.illumina.com for details of the sequencing technology.
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