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Accession IconSRP002245

Characterization of the RNA content of chromatin

Organism Icon Homo sapiens
Sample Icon 1 Downloadable Sample
Technology Badge IconIllumina Genome Analyzer

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We deep sequenced chromatin-associated RNAs (CARs) from human fibroblast (HF) cells. This resulted in the identification of 141 intronic regions and 74 intergenic regions harbouring CARS. Overall design: We purified CARs from normal HFs by isolating soluble chromatin after MNase treatment, followed by separation of chromatin fragments of different lengths on a sucrose gradient. CARs were converted into double-stranded cDNAs and sequenced using the Illumina Genome Analyzer I.
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