Transcriptome signature of WT and C3ar1-/-C5ar1-/- splenic DCs in response to CpG

Our group showed that DC-instrinsic C3ar1/C5ar1 signals are required for TLR-initiated DC maturation in vivo. To more broadly analyze how local complement signaling affects DC maturation process in response to TLR9 stimulation, WT or C3ar1-/-C5ar1-/- mice were stimulated with CpG (i.v. 100 micrograms) or vehicle control. 4hrs later, splenic CD11c+DCs were isolated and RNAs from the cells were purified for microarray analyses.

10

Sheen JH, Strainic MG, Liu J, Zhang W, Yi Z, Medof ME, Heeger PS