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Accession IconGSE85543

Transcriptional effects of soluble CD40 ligand on human nave B cells

Organism Icon Homo sapiens
Sample Icon 39 Downloadable Samples
Technology Badge Icon Affymetrix HT HG-U133+ PM Array Plate (hthgu133pluspm)

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We performed microarray analysis to derive gene signatures down-stream of soluble CD40 ligand stimulation in human naive B cells. Nave B cells were purified from healthy donor PBMC using negative selection beads (Miltenyi) and cultured with sCD40L at 2.5ug/ml for 6hr before microarray analysis. In the same study, cells were also harvested at day 5 post-stimulation to confirm sCD40L-induced B cell activation and proliferation. FACS analysis confirmed soluble CD40L induced up-regulation of CD86 and CD69 at 24hr. B cell proliferation was measured at day 4 post-stimulation by EdU incorporation.
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