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Accession IconGSE7658

Gene Expression Profile of Hematopoietic Stem Cells during Zebrafish Development

Organism Icon Danio rerio
Sample Icon 8 Downloadable Samples
Technology Badge Icon Affymetrix Zebrafish Genome Array (zebrafish)

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Description
In order to detect transcriptional differences between primitive and definitive hematopoietic stem and progenitor cells during regular development in the zebrafish embryo, gata1-GFP+/+(18 somites), lmo2-GFP+/+ (18 somites and 35 hpf)1 and cd41-GFP+/+ (35 hpf) cells from transgenic embryos were individually separated from GFP-/- cells by flow cytometry at the indicated stages. For each individual population, pools of 600 - 1500 transgenic embryos were collected. After RNA extraction, labelled cRNA was hybridized onto Affymetrix microarrays. Individual experiments were performed with 2 or 3 biological replicates.
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