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Accession IconGSE7010

Primary human epithelial cells exposed to coarse, fine and ultrafine particles

Organism Icon Homo sapiens
Sample Icon 11 Downloadable Samples
Technology Badge Icon Affymetrix Human Genome U133A Array (hgu133a)

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Description
The purpose of this study was to characterize global gene expression in human airway epithelial cells and identify cellular pathways associated with coarse, fine and ultrafine particulate matter (PM) exposures. Ambient PM was collected in 3 different size fractions from Chapel Hill air, particles were extracted from foam or filter matrices and lyophilized. Human primary airway epithelial cells were exposed to particles at 250g/ml or vehicle control for 6h in culture. Following exposure, RNA was isolated and hybridized to human HG U133A affymetrix chips.
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