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Accession IconGSE64468

Molecular mechanism of flocculation self-recognition in yeast and its role in mating and survival

Organism Icon Saccharomyces cerevisiae
Sample Icon 17 Downloadable Samples
Technology Badge Icon Affymetrix Yeast Genome 2.0 Array (yeast2)

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Saccharomyces cerevisiae flocculation occurs when fermentable sugars are limiting and is therefore considered as a way to enhance the survival chance of Flo-expressing yeast cells. In this paper, the role of Flo1p in mating was demonstrated by showing that the mating efficiency, which contributes to the increased survival rate as well by generating genetic variability, is increased when cells flocculate. This was revealed by liquid growth experiments in a low shear environment and differential transcriptome analysis of FLO1 expressing cells compared to the non-flocculent wild-type cells. The results show that a floc provides a uniquely organized multicellular ultrastructure that provides a suitable microenvironment to induce and perform cell conjugation.
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