github link
Accession IconGSE45857

Expression data from bovine liver

Organism Icon Bos taurus
Sample Icon 27 Downloadable Samples
Technology Badge Icon Affymetrix Bovine Genome Array (bovine)

Submitter Supplied Information

Description
Female sex steroid hormones, estradiol-17 (E2) and progesterone (P4) regulate reproductive function and gene expression in a broad range of tissues. Given the central role of the liver in regulating homeostasis including steroid hormone metabolism, we sought to understand how E2-17 and P4 interact to affect global gene expression in liver. Eight ovariectomized cows were randomly assigned to 4 treatment groups applied in a replicated Latin Square design: 1) No hormone supplementation, 2) E2-17 treatment (ear implant), 3) P4 treatment (intravaginal inserts), and 4) E2-17 combined with P4. After 14 d of treatment, liver biopsies were collected, allowing 28 d intervals between periods. Changes in gene expression in the liver biopsies were monitored using Affymetrix bovine-specific arrays. Treatment with E2-17 altered expression of 479 genes, P4 472 genes, and combined treatment significantly altered expression of 468 genes. In total, 578 genes exhibited altered expression including a remarkable number (346 genes) that responded similarly to E2-17, P4, or combined treatment. Additional evidence for similar gene expression effects of E2-17 and/or P4 were: principal component analysis placed almost every treatment array at a substantial distance from control arrays; Venn diagrams indicated overall treatment effects for most regulated genes; clustering analysis indicated the two major clusters had all treatments upregulating (cluster 1; 172 genes) or downregulating (cluster 2: 173 genes) expression. Thus, unexpectedly, common biological pathways are regulated by E2-17 and/or P4 in liver. Future studies are needed to elucidate mechanism(s) responsible for overlapping actions of E2-17 and P4 on the liver transcriptome. KEYWORDS: estradiol, progesterone, global gene expression, liver, cows.
PubMed ID
No associated PubMed ID
Publication Title
No associated publication
Total Samples
28
Submitter’s Institution
Authors
No associated authors

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Sex
Specimen part
Processing Information
Additional Metadata
No rows found
Loading...