Genome-wide gene expression analysis in Thoc5- and CFIm68-depleted HeLa cells

We found that CFIm68, a mRNA cleavage and polyadenylation factor implicated for alternative polyadenylation site choice, was co-purified with Thoc5, a component of human THO/TREX. Microarray analysis using human HeLa cells reveals knockdown of Thoc5 affects the expression of a subset of non-heat shock genes. Notably, depletion of Thoc5 attenuated the expression of the mRNAs polyadenylated at distal, but not proximal, polyadenylation sites, which phenocopied the depletion of CFIm68.

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Katahira J, Okuzaki D, Inoue H, Yoneda Y, Maehara K, Ohkawa Y