Expression profile in wild type, fkh2delta and fkh2-S2A mutants

Mitotic entry is accompanyed by the expression of a cluster of so called mitotic genes, whose activation is critical for mitosis in human and yeast cells. We found a link between the transcription machinery and cell cycle control network at mitosis in fission yeast, involving the Cdk8 kinase dependent phosphorylation of the fork head transcription factor Fkh2. We have generated a non-phosphorylatable fkh2 mutant (fkh2-S2A) also.

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Szilagyi Z, Banyai G, Lopez MD, McInerny CJ, Gustafsson CM