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Accession IconGSE27175

Formalin Fixation at Low Temperature Better Preserves Nucleic Acid Integrity

Organism Icon Homo sapiens
Sample Icon 30 Downloadable Samples
Technology Badge IconIllumina HumanHT-12 V3.0 expression beadchip

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INTRODUCTION. Fixation with formalin, a widely adopted procedure to preserve tissue samples, leads to extensive degradation of nucleic acids and thereby compromises procedures like microarray-based gene expression profiling. We hypothesized that RNA fragmentation is caused by activation of RNAses during the interval between formalin penetration and tissue fixation. To prevent RNAse activation, a series of tissue samples were kept under-vacuum at 4C until fixation and then fixed at 4C, for 24 hours, in formalin followed by 4 hours in ethanol 95%.
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