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Accession IconGSE26148

Expression profiling of MCF10A cells in 2D and 3D culture

Organism Icon Homo sapiens
Sample Icon 13 Downloadable Samples
Technology Badge Icon Affymetrix Human Genome U133A Array (hgu133a)

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In these microarray experiments, we characterize the gene expression of mammary epithelial cells (MCF10A cells) grown in either a traditional monolayer cell culture setting (2D) or on Matrigel, which induces single MCF10A cells to form organized acinar structures (3D). Morphogenesis of mammary epithelial cells into organized acinar structures in vitro is accompanied by widespread changes in gene expression patterns, including a substantial decrease in expression of Myc.
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