Identification of genes responsive to hyperthermia in human leukemia U937 cells

Hyperthermia (41C <) is widely used to treat patients with various cancers. Here, the effects of hyperthermia (42C for 90 min) on the gene expression in human lymphoma U937 cells were investigated using an Affymetrix GeneChip system. The cells were treated with hyperthermia (42C for 90 min) and followed by incubation for 0, 1, 3 or 6 h at 37C. The percentage of DNA fragmentation was 7.5 0.9 (mean SD), 10.1 0.2, and 17.3 2.3 at the incubation periods of 1, 3, and 6 h, respectively. Of approximately 47,000 probe sets analyzed, the hyperthermia down-regulated 4,214 probe sets and up-regulated 1,334 by a factor 2.0 or greater.

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Furusawa Y, Tabuchi Y, Takasaki I, Wada S, Ohtsuka K, Kondo T