github link
Accession IconGSE12991

Isolation of single miRNA-expressing cells from zebrafish embryos

Organism Icon Danio rerio
Sample Icon 18 Downloadable Samples
Technology Badge Icon Affymetrix Zebrafish Genome Array (zebrafish)

Submitter Supplied Information

Description
The goal of the project was to isolate single miRNA-expressing cells labelled by GFP reporter genes under the control of endogenous miRNA promoters and analyze expression levels of miRNA target genes in these cells. GFP-positive miRNA-expressing cells and GFP-negative cells from the rest of the embryos were purified at the same developmental stage to the cellular resolution using fluorescent activated cell sorting (FACS). Focus was on regulation by miR-206 and miR-133 in the developing somites and miR-124 in the developing central nervous system. Comparison of wild-type embryos and those lacking miRNAs revealed predicted
PubMed ID
Total Samples
18
Submitter’s Institution

Samples

Show of 0 Total Samples
Filter
Add/Remove
Accession Code
Title
Processing Information
Additional Metadata
No rows found
Loading...