Characterizing the gene expression profile of Prox1+ intestinal adenoma organoid cells

GSE117981

Mus musculus

6 Downloadable Samples

Illumina MouseWG-6 v2.0 expression beadchip

Submitter Supplied Information

Description

We isolated and selected intestinal adenoma organoids from Apcmin/+; Rosa26LSL-TdTomato; Prox1-CreERT2 mice. After the selection procedure without growth factors, we induced CreERT2 activity and the transcription of tdTomato to label Prox1+ cells by 300 nM 4-hydroxytamoxifen for 16h. tdTomato+ (Prox1+) and tdTomato- cells (enriched for Prox1- cells) were FACS sorted and total RNA was isolated.

PubMed ID

30154153

Publication Title

Transcription Factor PROX1 Suppresses Notch Pathway Activation via the Nucleosome Remodeling and Deacetylase Complex in Colorectal Cancer Stem-like Cells.

Total Samples

Submitter’s Institution

Semmelweis University

Authors

Högström J, Heino S, Kallio P, Lähde M, Leppänen VM, Balboa D, Wiener Z, Alitalo K

Source Repository

Gene Expression Omnibus (GEO)

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