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Accession IconERP005289

RNASeq analysis of embryonic day 13.5 mouse cerebral cortex from control and Pax6-deleted embryos

Organism Icon Mus musculus
Sample Icon 180 Downloadable Samples
Technology Badge IconIllumina HiSeq 2500

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Description
Mice carrying embryos that were either Pax6fl/fl;Emx1CreER (experimental group) or Pax6fl/+;Emx1CreER (control group) were given tamoxifen on embryonic day 9.5 (E9.5) to induce Pax6 deletion. Embryos were harvested on E13.5, the cerebral cortices were removed and divided into rostral and caudal halves, and total RNA was extracted. Samples from littermates of the same genotype were pooled. Poly-A mRNA was purified and TruSeq RNA-Seq libraries were prepared and sequenced (100 base paired-end; Illumina, HiSeq v3).
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