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Accession IconE-MTAB-3711

Identification of chicken Interferon Stimulated Genes (ISGs) using Affymetrix GeneChip Chicken Genome Arrays

Organism Icon Gallus gallus
Sample Icon 6 Downloadable Samples
Technology Badge Icon Affymetrix Chicken Genome Array (chicken)

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Description
Recombinant chicken IFN1 was prepared as previously reported (Laidlaw et al, 2013) and was added in chicken embryo fibroblasts to a final concentration of 1000 U/ml. Confluent cells were treated with chicken IFNa or mock treated and incubated for six hours before harvesting. The experiment was repeated in triplicate with three different batches of CEFs.
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