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Accession IconE-MTAB-2904

Collagen induces maturation of human monocyte-derived dendritic cells by signalling through Osteoclast Associated Receptor

Organism Icon Homo sapiens
Sample Icon 96 Downloadable Samples
Technology Badge Icon Affymetrix HT HG-U133+ PM Array Plate (hthgu133pluspm)

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Description
Human immature dendritic cells derived from monocytes were activated to become mature dendritic cells by exposure to platebound Collagen I or Collagen. The importance of OSCAR-signalling was evaluated by pre-incubating the immature dendritic cells with an inhibitory monoclonal antibody to the OSCAR receptor, or an isotype control. As a positive control, immature dendritic cells were activated by using LPS. The set-up was examined at 4 hours and 20 hours to evaluate early and late effects of OSCAR signalling.
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