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Accession IconE-MEXP-867

Transcription profiling by array of mouse pancreatic beta cells after treatment with glucose

Organism Icon Mus musculus
Sample Icon 4 Downloadable Samples
Technology Badge Icon Affymetrix Mouse Expression 430A Array (moe430a)

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Description
To identify proteins regulated by glucose through changes in their rate of protein synthesis, translational profiling of MIN6 cells acutely incubated at either low or high glucose concentration was performed (i.e. microarray analysis was performed on mRNAs associated with polysomes, as an increase in the association of mRNA with polysomes is indicative of an increase in the rate of initiation step of translation and hence an increase in protein expression) (Johannes et al., 1999; Mikulits et al., 2000).
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