Affymetrix GeneChip P. aeruginosa Genome[Pae_G1a]

Fosfomycin and tobramycin in combination downregulate nitrate reductase genes narG and narH, resulting in increased activity against P. aeruginosa under anaerobic conditions.

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The experiment aimed to characterise the transcriptional response of a clinical CF P. aeruginosa isolate to sub-inhibitory concentrations of fosfomycin, tobramycin and a novel 4/1 (w/w) combination of the two (FT) under both aerobic and anaerobic conditions

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Transcription profiling by array of Pseudomonas aeruginosa treated with fosfomycin, tobramycin or a combination of both drugs under aerobic and anaerobic conditions

Transcriptional response of P. aeruginosa to fosfomycin tobramycin and FT under aerobic and anaerobic conditions

The experiment aimed to characterise the transcriptional response of a clinical CF P. aeruginosa isolate to sub-inhibitory concentrations of fosfomycin, tobramycin and a novel 4/1 (w/w) combination of the two (FT) under both aerobic and anaerobic conditions


P-MTAB-29471

One millilitre of culture was withdrawn and 1ml of solution containing the appropriate concentration of antibiotic (F:T 1.25mg/L, fosfomycin 1mg/L, tobramycin 0.25mg/L and Control - sterile water) was added in triplicate under both aerobic and anaerobic conditions. The cultures were then incubated with shaking for a further one hour. 

Transcription profiling by array of Pseudomonas aeruginosa treated with fosfomycin, tobramycin or a combination of both drugs under aerobic and anaerobic conditions

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