Affymetrix GeneChip Mouse Expression Array MOE430A [MOE430A]

Macrophage Migration Inhibitory Factor: A Central Regulator of Wound Healing

rawData

scan

processedDataMatrix

The aim of this experiment was to investigate the role of MIF during wound healing using BALB/C MIF null mice and in the context of reduced estrogen-associated impaired healing using ovariectomized mice (a mouse model of age-associated delayed healing). Ageing is associated with delayed cutaneous wound healing resulting from reduced estrogen levels. Macrophage migration inhibitory factor (MIF - NCBI RefSeq: NM_010798) is thought to mediate the effects of estrogen on wound healing. Gene expression was compared between wounds from ovariectomized MIF null mice and controls.

Clinical Information

Genotype

Transcription profiling of wounds from ovariectomized MIF null mice and controls to investigate the role of MIF during wound healing using BALB/C MIF null mice

The aim of this experiment was to investigate the role of MIF during wound healing using BALB/C MIF null mice and in the context of reduced estrogen-associated impaired healing using ovariectomized mice (a mouse model of age-associated delayed healing). Ageing is associated with delayed cutaneous wound healing resulting from reduced estrogen levels. Macrophage migration inhibitory factor (MIF - NCBI RefSeq: NM_010798) is thought to mediate the effects of estrogen on wound healing. Gene expression was compared between wounds from ovariectomized MIF null mice and controls.


Affymetrix:Protocol:ExpressionStat

Title: Affymetrix CHP Analysis (ExpressionStat). Description:

Affymetrix:Protocol:Hybridization-EukGE-WS2v4[]

Title: Affymetrix EukGE-WS2v4 Hybridization. Description:

P-AFFY-6

Title: Affymetrix CEL analysis. Description:

TRANPRTCL2797

Expression analysis was performed using RMAExpress (02 release) using the background correction and quantile normalisation options. In addition, scaling normalisation (target signal 100) using Affymetrix MAS 5.1 was done for the .CHP files submitted to this database.

P-MEXP-6065

P-MEXP-6070

Temperature 20-21 C, Humidity 40-60%, Specified mouse pathogen free conditions, 12 hours light/dark cycle, Water ad libitum, Nutrients Rat and Mouse Standard Diet no.1 expanded (Ban Kingman), Housed in cages according to United Kingdom Home Office Regulations.

P-MEXP-6071

Ten week old female BALB/C strain mice were anaethetized with inhaled isoflourane, and the dorsum was shaved and cleaned with alcohol. Two equidistant 1cm full thickness skin incisional wounds were made through both skin and panniculus carnosus muscle and left to heal by secondary intention in accordance with UK Home Office regulations, under project licence 40/2526. Mice were administered buprenorphine 0.1mg/kg s/c as analgesia and housed individually. Three days following wounding mice were terminally anaethetized with chloroform and the wound tissue exised. Wounds were bisected and half processed for histology, while the remaining half was immediately frozen and stored at -80C prior to RNA extraction.

P-MEXP-6072

Ten week old female BALB/C strain macrophage migration inhibitory factor (Mif - NCBI:NM_010798) null mice were anaethetized with inhaled isoflourane, and the dorsum was shaved and cleaned with alcohol. Two equidistant 1cm full thickness skin incisional wounds were made through both skin and panniculus carnosus muscle and left to heal by secondary intention in accordance with UK Home Office regulations, under project licence 40/2526. Mice were administered buprenorphine 0.1mg/kg s/c as analgesia and housed individually. Three days following wounding mice were terminally anaethetized with chloroform and the wound tissue exised. Wounds were bisected and half processed for histology, while the remaining half was immediately frozen and stored at -80C prior to RNA extraction.

P-MEXP-6073

Six week old female BALB/C strain mice underwent bi-lateral ovariectomisation under inhaled halothane anesthesia with buprenorphine 0.1mg/kg s/c analgesia in accordance with UK Home Office regulations, under project licence 40/2526. Four weeks later mice were anaethetized with inhaled isolfourane, and the dorsum was shaved and cleaned with alcohol. Two equidistant 1cm full thickness skin incisional wounds were made through both skin and panniculus carnosus muscle and left to heal by secondary intention in accordance with UK Home Office regulations, under project licence 40/2526. Mice were administered buprenorphine 0.1mg/kg s/c as analgesia and housed individually. Three days following wounding mice were terminally anaethetized with chloroform and the wound tissue exised. Wounds were bisected and half processed for histology, while the remaining half was immediately frozen and stored at -80C prior to RNA extraction.

P-MEXP-6074

Six week old female BALB/C strain macrophage migration inhibitory factor (Mif - NCBI:NM_010798) null mice underwent bi-lateral ovariectomisation under inhaled halothane anesthesia with buprenorphine 0.1mg/kg s/c analgesia in accordance with UK Home Office regulations, under project licence 40/2526. Four weeks later mice were anaethetized with inhaled isolfourane, and the dorsum was shaved and cleaned with alcohol. Two equidistant 1cm full thickness skin incisional wounds were made through both skin and panniculus carnosus muscle and left to heal by secondary intention in accordance with UK Home Office regulations, under project licence 40/2526. Mice were administered buprenorphine 0.1mg/kg s/c as analgesia and housed individually. Three days following wounding mice were terminally anaethetized with chloroform and the wound tissue exised. Wounds were bisected and half processed for histology, while the remaining half was immediately frozen and stored at -80C prior to RNA extraction.

Transcription profiling of wounds from ovariectomized MIF null mice and controls to investigate the role of MIF during wound healing using BALB/C MIF null mice

Submitter Supplied Information

Samples