RNA-seq analysis of RPTEC under hypoxic condition with Dznep.

Hypoxia plays important roles in progression of chronic kidney diseases. HIF1 (hypoxia inducible factor 1) is a master transcriptional factor under hypoxic condition. To clarify the molecular mechanisms of HIF1 and identify novel linc RNAs under hypoxia, we performed RNA-seq using human renal proximal tubular epithelial cells (RPTEC). In addition, we use Dznep which is an inhibitor of H3K27me3 to examine the relationship between HIF1 and epigenetic modifiers under hypoxia.

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