RNA sequencing project of mouse

E13.5 Atm+/- pregnant mice were intraperitoneally injected with saline or etoposide 0.5 mg/kg consecutive three days, and scarified 24 hr after final injection. After genotyping, samples were subjected RNA sequencing. Total RNA was extracted from mice fetal liver. The libraries were then submitted for Illumina HiSeq1500 sequencing according to the standard operation. Paired-end 90 or 100 nucleotide reads were generated and checked for data quality using FASTQC. After etoposide treatment, various chimeric fusion mRNAs were observed in Atm wild type and deficient liver cells. The number of chimeric fusion mRNAs was much higher in Atm deficient fetal liver than wild type littermate. Mll-ptp4a2 fusion mRNA was identified from Atm deficient liver cells by etoposide exposure.

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